Journal: International Journal of Molecular Sciences

Article Title: Telomerase Inhibitor TMPyP4 Alters Adhesion and Migration of Breast-Cancer Cells MCF7 and MDA-MB-231

doi: 10.3390/ijms20112670

Figure Lengend Snippet: TMPyP4 triggers migration impairment in breast-cancer cells. MCF7 and MDA-MB-231 cells were incubated until reaching ca 70% confluence followed by a scratch with a sterile pipette tip. Samples were treated for 48 h with TMPyP4 (0.5 or 5 μM), and the wound healing properties were documented using a Zeiss Axiovert microscope (magnification 100 ×). Typical result out of 3 replicates was demonstrated.

Article Snippet: Photographs of the scratches were captured on day 0 and again 24, 48 and 72 h later under phase contrast microscope (Carl Zeiss, Oberkochen, Germany).

Techniques: Migration, Incubation, Sterility, Transferring, Microscopy

Journal: International Journal of Molecular Sciences

Article Title: Telomerase Inhibitor TMPyP4 Alters Adhesion and Migration of Breast-Cancer Cells MCF7 and MDA-MB-231

doi: 10.3390/ijms20112670

Figure Lengend Snippet: TMPyP4-mediated inhibition of adhesion properties of cancer cells. MCF7 and MDA-MB-231 cells were grown till reaching 70% confluence and exposed to 10, 20 or 50 μM TMPyP4 for 24 h. Then cells were trypsinized, and 5 × 10 5 cells were transferred to fresh dishes. Cells were analyzed under the phase contrast microscope and photographed after 15, 30, 60 min, 3 h and 18 h (Zeiss Axiovert microscope; magnification 100×). The most significant difference was observed after 18 h. (All studied time intervals were shown in the ). Typical result out of 3 replicates was demonstrated.

Article Snippet: Photographs of the scratches were captured on day 0 and again 24, 48 and 72 h later under phase contrast microscope (Carl Zeiss, Oberkochen, Germany).

Techniques: Inhibition, Microscopy